Study of Cytotoxic Effects of Saffron in MCF-7 Cells Cytotoxicity of saffron
Iranian Journal of Pharmaceutical Sciences,
Vol. 4 No. 4 (2008),
1 Mehr 2008
,
Page 261-268
https://doi.org/10.22037/ijps.v4.41052
Abstract
Current therapies for breast cancer are often limited by short-term efficacy due to the emergence of drug resistance. There has been increased interest in the use of naturally occurring compounds with chemopreventive and chemotherapeutic effects in the treatment of cancers. Saffron, dry stigmas Crocus sativusL., used in Iran as a spice, is known for its anticancer properties. In this study, the cytotoxic and apoptogenic effects of saffron in MCF-7 cells as an in vitromodel for breast cancer study were investigated. Meanwhile role of caspases were studied in its toxicity. MCF-7 and L929 cell lines were cultured in DMEM medium and incubated with different concentrations of saffron extract (100 to 2000 μg/ml). Cell viability was assessed by MTTassay. Apoptotic cells were determined using PI staining of DNA fragmentation by flow cytometry (sub-G1 peak). Role of caspase were studied using the pan-caspase inhibitor, z-VAD-fmk. Saffron extract decreased cell viability in MCF-7 cells as a concentration- and time-dependent manner. Doses of saffron inhibited 50% cell growth (IC50) against MCF-7 was 400 μg/ml after 48 h of incubation. Saffron could induce apoptosis in MCF-7 cells in which apoptosis was dependent on caspase activation. It might be concluded that saffron could cause MCF-7 cell death, in which apoptosis or programmed cell death play an important role.Saffron could be also considered as a promising chemotherapeutic agent in breast cancer treatment.
- Apoptosis
- Crocus sativus
- Cytotoxicity
- Saffron
How to Cite
References
[2]Goldstein CM, Mehren MV, Gradel T, Kilpatrick D, Vaders L, Brady D, Vogel L.Breast cancer research. Philadelphia: Fox Chase Cancer Center Scientific Report, 2000.
[3]Russo YX, Hu Y, Bove B, Huang Y, Silva IDCG,Tahin Q, Wuand HNY, Zekri A, Russo I.Biological and molecular basis of human breast cancer. Frontiers Biosci1998; 3: 944-60.
[4]Headand J, Johnston SR. New targets for therapy in breast cancer: Farnesyltransferase inhibitors.Breast Cancer Res2004; 6: 262-8.
[5]Amit KT, Madhumita R, Bhattacharya RK.Natural products as inducers of apoptosis:Implication for cancer therapy and prevention.Current Sci2001; 80: 1387-96
[6]Abdullaev FI. Biological effects of saffron.Biofactors1993; 4: 83-6.
[7]Abdullaev FI. Cancer chemopreventive and tumoricidal properties of saffron (Crocus sativusL.). Exp Biol Med2002; 227: 20-5.
[8]Abdullaev FI. Saffron (Crocus sativusL.) and its possible role in the prevention of cancer. In:Majumdar DK, Govil JN, Sing VK, (editors).Recent progress in medicinal plants. Houston:SCI Tech Publishing LLC, 2003; pp. 53-67.
[9]Abdullaev FI, Espinosa JJ, Aguirre. Biomedical properties of saffron and its potential use in cancer therapy and chemoprevention trials. Cancer Detection Prevention2004; 28: 426-32.
[10]Simstein R, Burow M, Parker A, Weldon C,Beckman B. Apoptosis, chemoresistance, and breast cancer: Insights from the MCF-7 cell model system. Exp Biol Med Maywood2003; 9: 995-1003.
[11]Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods983;65: 55-63.
[12]Sharifi AM, Mousavi SH, Bakhshayesh M,Tehrani FK, Mahmoudian M, Oryan S. Study of correlation between lead-induced cytotoxicity and nitric oxide production in PC12 cells. ToxicolLett2005; 160: 43-8.
[13]Zhang XD, Franco A, Myers K, Gray C, Nguyen T, Hersey P. Relation of TNF-related apoptosis-inducing ligand (TRAIL) receptor and FLICE-inhibitory protein expression to TRAIL-induced apoptosis of melanoma. Cancer Res1999; 1: 2747-53.
[14]Song SY, Lee I, Park C, Lee H, Hahm JC, Kikang W. Neolignans from Saururus chinensis inhibitPC-3 prostate cancer cell growth via apoptosis and senescence-like mechanisms. Int J Mol Med2005;16: 517-23.[15]Escribano J, Diaz-Guerra MJ, Riese HH, AlvarezA, Proenza R, Fernandez JA. The cytotoxic effect of glucoconjugate extracted from corms of saffron plant (Crocus sativus) on human cell lines in culture. Planta Med2002; 66: 157-62.
[16]Poncet KM, Kroemer G. Chemotherapy: Targeting the mitochondrial cell death pathway.Oncogene 2002; 21: 8786-803.
[17]Hersey P, Zhang XD. How melanoma cells evade trail-induced apoptosis. Nat Rev Cancer 2001; 1:142-50.
[18]Green DR, Reed JC. Mitochondria and apoptosis.Science1998; 281: 1309-12.
[19]Tripathi YB, Tripathi P, Arjmandi BH. Nutraceal and cancer management. Bioscience2005; 10:1607-18.
[20]Thornberry NA, Lazebnik Y. Caspases: Enemies within. Science1998; 281: 1312-6.
[21]Broker LE, Kruyt FA, Giaccone G. Cell death independent of caspases: Areview. Clin Cancer Res2005; 11: 3155-62.267
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