Determination of Vitamin C in Small Volumes of Blood by HPLC/EC Determination of vitamin C in blood
Iranian Journal of Pharmaceutical Sciences,
Vol. 1 No. 1 (2005),
15 January 2005
,
Page 7-10
https://doi.org/10.22037/ijps.v1.39436
Abstract
A sensitive procedure for determining total vitamin C (ascorbic acid + dehydrate ascorbic Acid) in a blood drop from a finger prick, before and after the administration of a vitamin C tablet is described. Analysis was carried out by high performance liquid chromatography with electrochemical detection (HPLC/EC).
Measurements were taken one hour, two hours and six hours after the administration of a 500 mg vitamin C tablet. D-Isoascorbic acid was used as the internal
standard and analysis was carried out using two C-18 columns connected in series and a phosphate buffer mobile phase. Dehydroascorbic acid in the samples was converted to ascorbic acid by incubation with DL-homocysteine for 30 minutes. The level of vitamin C in blood reached a maximum concentration after two
hours.
- Vitamin C
- Electrochemical detection
- HPLC
- Blood
How to Cite
References
[2] Tsao CS, Young M. A stabilized ascorbic acid solution. Med Sci Res 1996; 24: 473-475.
[3] Esteve MJ, Farre R, Frigola A, Garcia- Cantabella JM. Determination of ascorbic and dehydroascorbic acids in blood plasma and serum by liquid chromatography. J Chromatogr 1997; 688: 345-349.
[4] Vander Jagt DJ, Garry PJ, Hunt WC. Ascorbate in plasma as measured by liquid chromatography and by dichloroindophenol colorimetry. Clinic Chem 1986; 32: 1004-1006.
[5] Jogota SK, Dani HM. A new colorometric technique for the estimation of vitamin C using folin-phenol reagent. Anal Biochem 1982; 127: 178-182.
[6] Choi OK, Jo JS. Determination of ascorbic acid in foods by capillary zone electrophoresis. J Chromatogr1997; 781: 435-443.
[7] Manoharan M, Schwille PO. Measurement of ascorbic acid in human plasma and urine by high performance liuqid chromatography results in healthy-subjects and patients with idopathic calcium
urolithiasis. J Chromatogr 1994; 654: 134- 139.
[8] Iwase H, Ono I. Determination of ascorbic acid in food by column liquid chromatography with electrochemical detection using eluent for prerun sample stabilization. J Chromtogr 1998; 806: 361-364.
[9] Behrens WA, Madere R. Aprocedure for the separation and quantitative analysis of ascorbic acid, dehydroascorbic acid, isoascorbic acid and dehydroascorbic acid in food and animal tissue. J Liq Chromatogr 1994; 17: 2445- 2455.
[10] Graumlich JF, Ludden TM, Conry-Cantilena C, Cantilena LR Jr, Wang Y, Levine M. Pharmacokinetic model of ascorbic acid in healthy male volunteers during depletion and repletion. Pharm Res1997; 14: 1133-1139.
[11] Watson DG, Iqbal Z, Midgley JM, Pryce-Jones H, Morrison L, Dutton GN, Karditsas S, Wilson W. Measurement of ascorbic acid in human aqueous humour, plasma and bovine aqueous humour by high-performance liquid-chromatography with electrochemical detection. J Pharm Biomed Anal 1993; 11: 389-392.
[12] Bode AM, Rose RC. Analysis of water-soluble antioxidants by high-performance liquid chromatography with electrochemical detection. Methods Enzymol 1999; 299: 77-83.
- Abstract Viewed: 227 times
- IJPS_Volume 1_Issue 1_Pages 7-10 Downloaded: 201 times